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Pharmaceutical Raw Materials

Pharmaceutical Raw Materials

Stem Cell Culture Supernatant Stem- Sup™

Stem cell culture supernatant is a high-purity supernatant obtained in the process of culturing and proliferating Mesenchymal Stem Cells (MSCs) present in human-derived dental pulp, adipose, umbilical cord, bone marrow, etc.
Stem cells that make stem cell culture supernatant can be extracted from dental pulp of deciduous teeth, body fat, umbilical cord, etc., and their regenerative power differs depending on their origin.
Stem cells in culture release large amounts of various types of active substances called "bioactive substances" that involve in cell proliferation, angiogenesis, and immune regulation necessary for tissue repair.

  • Dental pulp-derived stem cell culture supernatant
    (Stemsup™️-DP)
  • Adipose-derived stem cell culture supernatant
    (Stemsup™️-A)
  • Umbilical cord-derived stem cell culture supernatant
    (Stemsup™️-Uc)
幹細胞培養上清

With aging, various tissues and functions of our body decline, such as weakened immunity and increased spots and wrinkles.
Proteins called growth factors and cytokines contained in human stem cell culture supernatant "StemSup®" have a role of recovering wounds and functions of tissues and cells in the body, and are expected to have various effects in the fields of internal medicine, dermatology, orthopedics, etc. (*Quoted from Biotherapy Institute of Japan, Inc.)


Commitment to the manufacture of StemSup®

The human stem cell culture supernatant "StemSup®" provided by our company is manufactured as a culture supernatant that can be used with peace of mind by conducting rigorous and multiple tests at one of the leading cell processing facilities in Japan. (*Quoted from Biotherapy Institute of Japan, Inc.)

Manufacturing scene of Stem Sup®

Point1: Raw materials limited to Japanese donors

In order to produce the human stem cell culture supernatant "StemSup®", there are strict criteria from the selection of donors for collecting stem cells to be cultured.
The raw materials (human adipose tissue, human dental pulp, human umbilical cord) are provided free of charge by healthy Japanese donors with the consent to manufacture StemSup® in collaboration with medical institutions in Japan.

Point2: Manufacture in a strictly controlled facility in Japan

Biotherapy Institute of Japan, Inc. is a Cell Processing Facility approved by the Minister of Health, Labor and Welfare, and has contributed to regenerative medicine and cell medicine for over 17 years.
Based on such abundant achievements and cell culture technology, the culture supernatant is also manufactured in a strictly controlled facility.

Point3: High inspection standards that support safety

We provide products that have passed general bacterial / fungal tests, endotoxin tests, mycoplasma tests, and more than 15 types of virus tests so that you can purchase the culture supernatant "StemSup® with peace of mind. In addition, there are cases where considerable amount of bovine-derived components are mixed in the culture supernatant on the market, but we confirm that these impurities are not contained in our products for each production lot.

Point4: High quality by optimized original culture technology

Currently, relatively inexpensive overseas-made culture supernatants are on the market, but the quality and quantity of useful bioactive substances such as growth factors and cytokines secreted in the culture supernatant vary greatly depending on the culture technology.

(*Quoted from Biotherapy Institute of Japan, Inc.)

StemSup® is cultivated in a unique optimized system in Biotherapy Institute of Japan, Inc. Therefore, compared to other companies' culture supernatant, StemSup® contains 2 to 5 times more powerful growth factor (HGF) that promotes tissue growth and regeneration, and 10 to 100 times more exosomes that induce cell responses such as cell proliferation and function expression and promote tissue repair. (*Quoted from Biotherapy Institute of Japan, Inc.)

比較

We measure the bioactive substances contained in each production lot in StemSup® manufacturing and provide only products that meet high quality standards.
We use stem cells for StemSup® production only to make StemSup® in better condition and discard the cells after collecting the supernatant.(*Quoted from Biotherapy Institute of Japan, Inc.)

StemSup EVs Analysis of StemSup EVs surface markers]
Exosome quantification (CD63)
StemSup EVs Electron micrograph

Comparison of the amount of bioactive substances and bovine albumin contamination in StemSup-A, -DP and other companies' products

Human Hepatocyte Growth Factor (HGF)) Vascular Endothelial Growth Factor (VEGF) Exosome Transforming Growth Factor-β1 (TGF-β1) Bovine albumin (contaminant))

StemSup™ -DP contains a large amount of "Vascular Endothelial Growth Factor (VEGF)" and "Transforming Growth Factor-β1 (TGF-β1)". StemSup™ -A contains a very large amount of "Human Hepatocyte Growth Factor (HGF)". In addition, you can see that both StemSup™ -A and StemSup™ -DP contain many "exosomes".


Dental pulp-derived stem cell culture supernatant

Dental pulp-derived stem cell culture supernatant

Dental pulp-derived stem cell culture supernatant is the supernatant obtained in the process of collecting and culturing the cells of a child's deciduous teeth.
Regeneration factors of dental pulp-derived stem cell are highly effective in many ways and have excellent proliferative capacity. Among them, "deciduous dental pulp-derived stem cells" are said to have the strongest regenerative power.

New regenerative medicine with Growth Factor

It is a new treatment method in which "growth factors" secreted into the "culture supernatant" of stem cells are administered instead of administering the stem cells themselves.

Expected effects

Intravenous administration

  • Improvement of diabetes, diabetic peripheral neuropathy, and diabetic nephropathy
  • Improvement of liver dysfunction
  • Improvement of peripheral neuropathy due to cervical spondylosis and lumbar spondylosis
  • Improvement of tiredness, stiff shoulders, etc.
  • Promotion of hair growth and hair restoration
  • Antioxidant effect on the skin
  • Improvement and prevention of wrinkle
  • Anti-aging effect, etc.

Adipose-derived stem cell culture supernatant

Adipose-derived stem cell culture supernatant

Adipose-derived stem cell culture supernatant is the supernatant obtained in the process of culturing the stem cells present in the adipose tissue collected by suction from the abdomen.
Adipose-derived stem cell culture supernatant is rich in bioactive substances that help tissue regeneration. It is a humoral factor made by culturing stem cells existing in adipose tissue that is aspirated from the abdomen, etc., and collecting and purifying the culture supernatant (liquid part that does not contain cells).
This culture supernatant is rich in TGF-β and other hundreds of cytokines and interleukins secreted by stem cells.

Expected effects

Intravenous administration

  • Anti-aging
  • Recovery of fatigue
  • Recovery of male functional
  • Recovery of sight
  • Improvement of sleep disorder
  • Improvement of blood circulation

Intradermal injection / application / nasal drip

  • Cerebral infarction
  • Spinal cord injury
  • Multiple sclerosis
  • Hypoxic ischemic encephalopathy
  • Parkinson's disease
  • Alzheimer's disease
  • Peripheral neuropathy
  • Fulminant hepatitis
  • Chronic hepatitis
  • Liver cirrhosis
  • Bone defect
  • Osteoporosis
  • Periodontal disease
  • Arthritis
  • Interstitial pneumonia
  • Ischemic heart disease
  • Diabetes mellitus
  • Diabetic nephropathy
  • Diabetic neuropathy
  • Allergic dermatitis
  • Alopecia

Umbilical cord-derived stem cell culture supernatant

Umbilical cord-derived stem cell culture supernatant

Umbilical cord mesenchymal stem cells are said to be more involved in tissue repair, immune control, tissue healing, etc., as compared to bone marrow-derived, adipose-derived, or deciduous dental pulp-derived mesenchymal stem cells.
In addition, it has been confirmed that various production substances secreted from umbilical cordderived stem cells contain cytokines and exosomes, and promote the production of collagen and hyaluronic acid to skin fibroblasts

Comparison table of microRNAs in exosomes of umbilical cord-derived mesenchymal stem cells

Comparison table of microRNAs in exosomes of umbilical cord-derived mesenchymal stem cells

Expected effects

Disease models with reported good results

Diseases with reported effectiveness in animal models

  • Leukemia
  • Cerebral palsy
  • Parkinson's disease
  • Osteoporosis
  • Diabetes mellitus
  • Aplastic anemia
  • Spinal cord injury
  • Chronic hepatitis
  • Diabetic nephropathy
  • Alopecia
  • Rheumatoid arthritis
  • Bone defect
  • Multiple sclerosis
  • Liver cirrhosis
  • Arthritis
  • Interstitial pneumonia
  • Atopic dermatitis
  • Heart disease